| DOI | Resolve DOI: https://doi.org/10.1007/978-1-0716-0795-4_28 |
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| Author | Search for: Qian, Hui1; Search for: Jia, Yimei2; Search for: McCluskie, Michael J.2 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
- National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Book Chapter |
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| Subject | Cyro-TEM; plunge freezing; electron tomography; immunogold labeling; vaccine delivery carriers |
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| Abstract | Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D). |
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| Publication date | 2020-09-22 |
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| Publisher | Humana |
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| In | |
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-HHT_53512 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | dcd68da0-a427-4013-bfa8-a69e54e62162 |
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| Record created | 2021-01-09 |
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| Record modified | 2021-01-09 |
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