Application of cryogenic transmission electron microscopy for evaluation of vaccine delivery carriers

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/978-1-0716-0795-4_28
AuthorSearch for: 1; Search for: 2; Search for: 2
Name affiliation
  1. National Research Council of Canada. Nanotechnology
  2. National Research Council of Canada. Human Health Therapeutics
FormatText, Book chapter
Book titleVaccine Delivery Technology
Series titleMethods in Molecular Biology; Volume 2183
ISSN1064-3745
1940-6029
ISBN978-1-0716-0794-7
978-1-0716-0795-4
Pages499–511
SubjectCyro-TEM; plunge freezing; electron tomography; immunogold labeling; vaccine delivery carriers
Abstract
Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D).
Publication date
PublisherHumana
LanguageEnglish
Peer reviewedYes
NRC numberNRC-HHT_53512
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Record created2021-01-09
Record modified2021-01-09
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