National Research Council of Canada. NRC Institute for Biodiagnostics
adenylate kinase; short variant; Mycobacterium tuberculosis; NMR spectroscopy; infrared spectroscopy
The adk gene from Mycobacterium tuberculosis codes for an enzyme of 181 amino acids. A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of “short” bacterial AKs. The recombinant protein, overexpressed in Escherichia coli, exhibits a low catalytic activity and an unexpectedly high thermal stability (Tm = 64.8°C). Based on various spectroscopic data, on the known three-dimensional structure of the AK from E. coli and on secondary structure predictions for various sequenced AKs, we propose a structural model for AK from M. tuberculosis (AKmt).
Proteins: Structure, Function, and Bioinformatics36, no. 2: 238–248.