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A reliable method for extraction of RNA from various conifer tissues

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00232985
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectconifer; Picea glauca; RNA extraction
Abstract
A simple and efficient procedure suitable for extraction of high-quality RNA from cultured conifer tissues, somatic embryos, zygotic embryos, needles, stem and root tissues was developed. It produced from 100 μg up to 700 μg total RNA per gram tissue dependent on the types of tissues used. RNA quality was estimated by spectrophotometry, agarose gel electrophoresis, in vitro translation of mRNA, cDNA synthesis and Northern blot analysis. The method also worked well with Arabidopsis thaliana and tobacco tissues.
Publication date
PublisherSpringer Nature
In
  • Plant Cell Reports 15, no. 7 (March 1996): 516–521.
LanguageEnglish
Peer reviewedYes
IdentifierBF00232985
NRCC-38457
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Record created2023-11-02
Record modified2023-11-02
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