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Structural and immunochemical characterization of the type VIII group B Streptococcus capsular polysaccharide

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FormatText, Article
Subject13C-NMR; 1H-NMR; acids; antigens; antigens, bacterial; bacterial; bacterial capsules; Canada; capsular polysaccharide; capsule; carbohydrate sequence; chemistry; d-glucose; group B Streptococcus; high-resolution; immunochemical characterization; immunology; magnetic resonance spectroscopy; Meningitis; molar; molecular sequence data; Neuraminidase; NMR spectroscopy; nuclear magnetic resonance; oxidation-reduction; polysaccharides; repeating unit; resolution; resonance; sialic acids; strains; Streptococcus; Streptococcus agalactiae; structural; structure; support, U.S. Gov't, P.H.S.; unit
Abstract
The type VIII capsular polysaccharide has been isolated and purified from a newly described strain of group B Streptococcus which is a leading cause of sepsis and neonatal meningitis in Japan. The polysaccharide contains D-glucose, D-galactose, L-rhamnose, and sialic acid in the molar ratio 1:1:1:1. By means of high resolution 1H nuclear magnetic resonance (1H NMR), 13C NMR, and homo- and heterocorrelated NMR, the repeating unit structure of the type VIII polysaccharide was delineated as the following, [formula: see text] Enzymatic studies established this polysaccharide as the first from which sialic acid, linked to a branched beta-D-galactopyranosyl residue, is known to be removed by bacterial neuraminidase
Publication date
PublisherAmerican Society for Biochemistry and Molecular Biology
In
  • The Journal of Biological Chemistry 271, no. 15: 8786–8790.
LanguageEnglish
Peer reviewedYes
NRC numberKOGAN1996
NPARC number9364359
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Record identifierd70dd315-1daf-4e1a-ae56-d58768d6b8ba
Record created2009-07-10
Record modified2020-03-20
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