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Endochitinase and β-1,3-glucanase genes are developmentally regulated during somatic embryogenesis in Picea glauca

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF01007703
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectabscisic acid; endochitinase; fungal elicitation; β-1,3-Glucanase; Picea glauca; somatic embryogenesis
Abstract
Two cDNAs isolated from white spruce [Picea glauca (Moench) Voss] somatic embryos, are predicted to encode a basic class IV chitinase and a β-1,3-glucanase, respectively corresponding to genesPgChi-1 andPgGlu-1. Each represents a multigene family in spruce. Transcripts homologous toPgChi-1 orPgGlu-1 genes were highly abundant in embryogenic tissues and gradually decreased after tissues were placed on abscisic acid-containing maturation medium, with lowest abundance in globular embryos. Transcripts related toPgGlu-1 became highly abundant again in early cotyledonary embryos but decreased thereafter, whereas transcripts related toPgChi-1 were also highly abundant in late cotyledonary embryos and plantlets in vitro; transcripts were either low (PgChi-1) or were not detectable (PgGlu-1) in needles. Wounding, drying and flooding stresses enhancedPgChi-1-andPgGlu-1-related gene expression. Fungal cell wall suspension enhancedPgGlu-1-related transcript accumulation, but reducedPgChi-1-related transcript abundance within 24 h.PgChi-1 andPgGlu-1 and their homologues may have roles in plant defense, and possibly developmental roles during spruce somatic embryo maturation.
Publication date
PublisherSpringer Link
In
  • Planta 201, no. 2 (1 June 1997): 189–194.
LanguageEnglish
Peer reviewedYes
IdentifierBF01007703
NRCC-40024
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Record created2023-11-01
Record modified2023-11-01
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