| Download | - View final version: Advances in gene editing of haploid tissues in crops (PDF, 1.5 MiB)
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| DOI | Resolve DOI: https://doi.org/10.3390/genes12091410 |
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| Author | Search for: Bhowmik, Pankaj1; Search for: Bilichak, AndriyORCID identifier: https://orcid.org/0000-0003-1281-504X |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | doubled haploid; gene editing; CRISPR/Cas9; haploid induction |
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| Abstract | Emerging threats of climate change require the rapid development of improved varieties with a higher tolerance to abiotic and biotic factors. Despite the success of traditional agricultural practices, novel techniques for precise manipulation of the crop’s genome are needed. Doubled haploid (DH) methods have been used for decades in major crops to fix desired alleles in elite backgrounds in a short time. DH plants are also widely used for mapping of the quantitative trait loci (QTLs), marker-assisted selection (MAS), genomic selection (GS), and hybrid production. Recent discoveries of genes responsible for haploid induction (HI) allowed engineering this trait through gene editing (GE) in non-inducer varieties of different crops. Direct editing of gametes or haploid embryos increases GE efficiency by generating null homozygous plants following chromosome doubling. Increased understanding of the underlying genetic mechanisms responsible for spontaneous chromosome doubling in haploid plants may allow transferring this trait to different elite varieties. Overall, further improvement in the efficiency of the DH technology combined with the optimized GE could accelerate breeding efforts of the major crops. |
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| Publication date | 2021-09 |
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| Publisher | MDPI |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | genes12091410 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ccaa4a27-e039-453b-bf0c-83427844ae7c |
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| Record created | 2021-10-15 |
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| Record modified | 2021-10-15 |
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