Digital in-line holography: 4-D imaging and tracking of micro-structures and organisms in microfluidics and biology

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1117/12.667447
Author	Search for: Garcia-Sucerquia, J.; Search for: Xu, W.; Search for: Jericho, S. K.; Search for: Jericho, M. H.; Search for: Tamblyn, I.; Search for: Kreuzer, H. J.
Editor	Search for: Von Bally, Gert; Search for: Luo, Qingming
Format	Text, Article
Conference	ICO20: Biomedical Optics, 21 August 2005, Changchun, China
Abstract	In recent years, in-line holography as originally proposed by Gabor, supplemented with numerical reconstruction, has been perfected to the point at which wavelength resolution both laterally and in depth is routinely achieved with light by using digital in-line holographic microscopy (DIHM). The advantages of DIHM are: (1) simplicity of the hardware (laser- pinhole-CCD camera), (2) magnification is obtained in the numerical reconstruction, (3) maximum information of the 3-D structure with a depth of field of millimeters, (4) changes in the specimen and the simultaneous motion of many species, can be followed in 4-D at the camera frame rate. We present results obtained with DIHM in biological and microfluidic applications. By taking advantage of the large depth of field and the plane-to-plane reconstruction capability of DIHM, we can produce 3D representations of the paths followed by micron-sized objects such as suspensions of microspheres and biological samples (cells, algae, protozoa, bacteria). Examples from biology include a study of the motion of bacteria in a diatom and the track of algae and paramecium. In microfluidic applications we observe micro-channel flow, motion of bubbles in water and evolution in electrolysis. The paper finishes with new results from an underwater version of DIHM.
Publication date	2006-02-02
Publisher	Society of Photo-Optical Instrumentation Engineers
In	ICO20: Biomedical Optics, 602613.
Series	Proceedings of SPIE.
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	23003175
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Record identifier	cbb880df-5e22-423e-aa86-fc494d62feb5
Record created	2018-05-03
Record modified	2020-04-22

Date modified:: 2025-12-17