Microcalorimetry as a diagnostic and analytical tool for the assessment of biodegradation of 2,4-D in a liquid medium and in soil

From National Research Council Canada

DOIResolve DOI: http://doi.org/10.1007/BF00902753
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Name affiliation
  1. National Research Council Canada
TypeArticle
Journal titleApplied Microbiology and Biotechnology
ISSN0175-7598
1432-0614
Volume42
Issue2-3
Pages432–439
Abstract
The biodegradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) byPseudomonas cepacia was assessed by microcalorimetry in a liquid medium and in sterilized soil at 25°C under aerobic conditions. It was found that thermograms of the rate of heat evolved versus time (dQ/dt versust) can be used as a diagnostic tool to identify the timet1 required for the primary biodegradation of 2,4-D and the timetf required for the completion of the biodegradation activity in a liquid medium as well as in soil. Microcalorimetry can also be used as an analytical tool to monitor the progress of 2,4-D consumption during the biodegradation process in a liquid medium and to measure the importance of the soil sorption/desorption of intermediate metabolites. A new concept called “bioeffort” was defined as the product of the biodegradation time (t) and the biomass concentration (X) at that time. This concept was used to predict either the biomass concentration required or the duration of the primary biodegradation of 2,4-D in soil from the data obtained from a liquid medium.
Publication date
PublisherSpringer
LanguageEnglish
Peer reviewedYes
NPARC number23001656
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