| DOI | Resolve DOI: https://doi.org/10.1016/0304-4211(74)90097-2 |
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| Author | Search for: Kartha, K. K.1; Search for: Michayluk, M. R.1; Search for: Kao, K. N.1; Search for: Gamborg, O. L.1; Search for: Constabel, F.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | Protoplasts from mesophyll cells of rape plants (Brassica napus L. cv. Zephyr) were isolated by enzymatic removal of cell walls in an osmoticum consisting of sorbitol and mannitol. A two-step enzyme treatment involving 0.5% each of Onozuka P1500 cellulase and Rhozyme HP150 hemicellulase followed by 0.5% each of Driselase and hemicellulase at pH 6.2 resulted in the production of viable protoplasts in 80–90% yield. The protoplasts were cultured in droplets of B5 medium in petri dishes at a light intensity of 100 lux and 26°. They were able to regenerate cell walls. Cell division was apparent after 30 h of culturing and by 60 h up to 38% of the protoplasts had divided once. Cell clusters were formed within 15 days of culturing. The calli originating from cell clusters were induced to differentiate and to form complete plants. |
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| Publication date | 1974-10 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | 0304421174900972 |
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| NRC number | NRCC-14034 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c470f91e-aa3d-488f-b0ab-1a2954126b01 |
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| Record created | 2023-07-06 |
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| Record modified | 2023-07-11 |
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