| DOI | Resolve DOI: https://doi.org/10.2166/wst.2000.0539 |
|---|
| Author | Search for: Hawari, J.1; Search for: Shen, C. F.1; Search for: Guiot, S. R.1; Search for: Greer, C. W.1; Search for: Rho, D.1; Search for: Sunahara, G.1; Search for: Ampleman, G.; Search for: Thiboutot, S. |
|---|
| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
|
|---|
| Format | Text, Article |
|---|
| Conference | International Conference on Waste Minimisation and End of Pipe Treatment in Chemical and Petrochemical Industries (IAWQ 1999), Nov. 14-18, 1999, Yucatan, Mexico |
|---|
| Subject | crossgroup; bioremediation; explosives; intermediates; mineralization; P. chrysosporium; Rhodococcus; sludge |
|---|
| Abstract | When TNT (N-source) was treated with anaerobic sludge it biotransformed into triaminotoluene (TAT) in high yield (80%). Results of experiments using ¹³C-labeling indicate that denitrated or deaminated products such as p-cresol and toluene were not formed. Whereas ¹⁴C-labeling showed negligible mineralization (<0.1% 14CO₂) despite the complete disappearance of TNT. On the other hand, when TNT (175μM) was treated with the fungus Phanerochaete chrysosporium it disappeared completely in less than two weeks, but mineralization (liberated ¹⁴CO₂) did not exceed 1%. Several intermediates, marked with the initial formation of the two monohydroxylamino-dinitrotoluene (HADNT) followed by their transformation to monoamino-dinitrotoluenes (ADNT), diamines (DANT), acetylated TNT products, and azo and hydrazo derivatives were detected. In contrast, high concentrations (ca 20,000 ppm) of RDX and HMX were effectively degraded (ca 70%) in soil slurries using municipal anaerobic sludge. RDX and HMX disappearance was accompanied by the elimination of toxicity associated with RDX and HMX as determined by the Microtox test. |
|---|
| Publication date | 2000-09 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | CPENV20
43319 |
|---|
| NPARC number | 3539845 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | c39c27a4-ce49-4cfc-8ee2-29d53f1c9a66 |
|---|
| Record created | 2009-03-01 |
|---|
| Record modified | 2022-10-12 |
|---|
