| DOI | Resolve DOI: https://doi.org/10.1016/j.phytol.2015.03.003 |
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| Author | Search for: Taylor, Wesley G.; Search for: Sutherland, Daniel H.; Search for: Zhang, Haixia1; Search for: Hegedus, Dwayne D. |
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| Name affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | arginine; asparagine; aspartic acid; chymotrypsin; cysteine; disulfide; flavone; genomic DNA; glutamic acid; lentil extract; microsomal aminopeptidase; octapeptide; PA1b peptide; peptide; plant extract; proline; pronase; quinestrol; serine; trypsin; tyrosine; alkylation; amino acid sequence; amino terminal sequence; carboxy terminal sequence; collisionally activated dissociation; high performance liquid chromatography; hydrolysis; legume; lentil; matrix assisted laser desorption ionization time of flight mass spectrometry; nucleotide sequence; open reading frame; plant gene; soybean; Glycine max; Lens culinaris; Pisum sativum |
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| Abstract | Mature seeds of lentil (Lens culinaris Medik.) were previously reported to contain an insecticidal cysteine-rich peptide, likely of the albumin-1 subunit b type. The purpose of this work was to determine the amino acid sequence of this insecticidal lentil peptide in an Eston lentil extract by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), after reduction of the disulfide bridges, alkylation of the cysteine residues and hydrolysis by pronase, trypsin, chymotrypsin and endoproteinase Asp-N. Sequences of key fragments were supported by monoisotopic mass measurements and by sequence ions from collision-induced dissociation (CID) experiments with a MALDI-TOF/TOF analyzer (MS/MS analysis). The new 37 amino acid sequence revealed strong similarities to a histidine-containing pea PA1b peptide and to soybean leginsulins but with a unique segment of RSSA in the middle. The lentil PA1b peptide sequence agreed completely with that derived from a L. culinaris genomic DNA sequence. © 2015 Phytochemical Society of Europe. |
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| Publication date | 2015-03-19 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21275720 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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| Record identifier | c10d14ab-2f95-4a1d-9617-d98cbea6f87f |
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| Record created | 2015-07-14 |
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| Record modified | 2020-04-22 |
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