| DOI | Resolve DOI: https://doi.org/10.1128/EC.00387-06 |
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| Author | Search for: Dignard, Daniel1; Search for: El-Naggar, Ahmed L.; Search for: Logue, Mary E.; Search for: Butler, Geraldine; Search for: Whiteway, Malcolm1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | acids; amino acids; biotechnology; candida; Candida albicans; cells; genes; genome; genome sequence; pharmaceutical; protein; proteins; Saccharomyces cerevisiae |
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| Abstract | In the opaque state, MTLa and MTLalpha strains of Candida albicans are able to mate, and this mating is directed by a pheromone-mediated signaling process. We have used comparisons of genome sequences to identify a C. albicans gene encoding a candidate a-specific mating factor. This gene is conserved in Candida dubliniensis and is similar to a three-gene family in the related fungus Candida parapsilosis but has extremely limited similarity to the Saccharomyces cerevisiae MFA1 (ScMFA1) and ScMFA2 genes. All these genes encode C-terminal CAAX box motifs characteristic of prenylated proteins. The C. albicans gene, designated CaMFA1, is found on chromosome 2 between ORF19.2165 and ORF19.2219. MFA1 encodes an open reading frame of 42 amino acids that is predicted to be processed to a 14-amino-acid prenylated mature pheromone. Microarray analysis shows that MFA1 is poorly expressed in opaque MTLa cells but is induced when the cells are treated with alpha-factor. Disruption of this C. albicans gene blocks the mating of MTLa cells but not MTLalpha cells, while the reintegration of the gene suppresses this cell-type-specific mating defect |
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| Publication date | 2007 |
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| In | |
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| Language | English |
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| NRC number | 47551 |
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| NPARC number | 3540322 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | bc9aa966-150c-4113-8891-8fbd8499068a |
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| Record created | 2009-03-01 |
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| Record modified | 2020-05-10 |
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