| DOI | Resolve DOI: https://doi.org/10.1385/1-59259-356-9:269 |
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| Author | Search for: Nantel, André1 |
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| Editor | Search for: M. Terrian, David |
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| Name affiliation | - National Research Council Canada. NRC Biotechnology Research Institute
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| Format | Text |
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| Type | Book chapter |
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| Book title | Cancer Cell Signaling: Methods and Protocols |
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| Series title | Methods in Molecular Biology; Volume 218 |
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| ISBN | 978-1-58829-075-5 |
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| Pages | 269–276 |
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| Subject | pharmaceutical; cancer research; protein; protein-protein interactions; mutagenesis; screening; two-hybrid; interaction trap |
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| Abstract | Acquiring functional knowledge from protein-protein interaction studies often necessitates the production of binding-impaired mutants and the study of their effects in biological systems. In many cases, multiple ligands compete for binding to the same protein domain and it becomes useful to produce specific mutations that prevent binding to one ligand but not to the others. We have combined PCR mutagenesis and a two hybrid mating assay to produce a screening strategy that has already proven useful in isolating ligand-specific mutants of the Grb10 and Raf-1 signaling proteins. |
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| Publication date | 2003 |
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| Publisher | Humana Press Inc. |
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| Language | English |
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| NRC number | NRC-BRI-44804 |
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| NPARC number | 3539165 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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Record identifier | bc13845f-8e84-4666-8bdc-a42d7fede545 | | Record created | 2009-03-01 |
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| Record modified | 2020-03-03 |
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