| Download | - View accepted manuscript: Development of a SYBR safe TM technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays (PDF, 594 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.mimet.2008.01.016 |
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| Author | Search for: Martineau, Christine1; Search for: Whyte, Lyle G.; Search for: Greer, Charles W.1 |
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| Affiliation | - National Research Council Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | environmental; stable isotope probing; cesium chloride density gradient; SYBR safe™; ethidium bromide |
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| Abstract | SYBR safe™, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of ¹²C- and ¹³C-labelled DNA using SYBR safe™ gave similar results to those obtained using EtBr with pure cultures and environmental samples exposed to a ¹³C-labelled substrate, while the detection limit of DNA was enhanced by the use of SYBR safe™ by at least 5 times. The results demonstrated that SYBR safe™ is a safe, sensitive and effective alternative to the use of ethidium bromide in CsCl density gradients for DNA-SIP assays. |
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| Publication date | 2008-02-14 |
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| In | |
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| Language | English |
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| NRC number | NRCC 49950 |
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| NPARC number | 12465408 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | badd1c5b-cc10-478e-9c09-46cc5f6de442 |
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| Record created | 2009-10-26 |
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| Record modified | 2020-04-15 |
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