Activity of human Δ5 and Δ6 desaturases on multiple n-3 and n-6 polyunsaturated fatty acids

DOI	Resolve DOI: https://doi.org/10.1016/S0014-5793(01)03135-0
Author	Search for: de Antueno, Roberto J.; Search for: Knickle, Leah C.; Search for: Smith, Heidi; Search for: Elliot, Michele L.; Search for: Allen, Stephen J.; Search for: Nwaka, Solomon; Search for: Winther, Michael D.
Format	Text, Article
Journal title	FEBS Letters
ISSN	0014-5793 1873-3468
Volume	509
Issue	1
Pages	77–80
Abstract	Yeast co-expressing human elongase and desaturase genes were used to investigate whether the same desaturase gene encodes an enzyme able to desaturate n-3 and n-6 fatty acids with the same or different carbon chain length. The results clearly demonstrated that a single human Δ5 desaturase is active on 20:3n-6 and 20:4n-3. Endogenous Δ6 desaturase substrates were generated by providing to the yeast radiolabelled 20:4n-6 or 20:5n-3 which, through two sequential elongations, produced 24:4n-6 and 24:5n-3, respectively. Overall, our data suggest that a single human Δ6 desaturase is active on 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3.
Publication date	2001-11
Publisher	Wiley
Language	English
Peer reviewed	Yes
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	23000942
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Record identifier	b9dfdeb5-15f6-4e91-a2c7-3a75799cb649
Record created	2016-11-18
Record modified	2020-03-27