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At-line monitoring of bioreactor protein production by surface plasmon resonance

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/bit.21725
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Affiliation
  1. National Research Council Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectat-line protein monitoring; bioreactor protein production; HEK 293 cells; surface plasmon resonance; transient transfection
Abstract
An innovative and automated method for the at-line monitoring of secreted protein was developed by harnessing a Surface Plasmon Resonance-based biosensor to a bioreactor. The proof of concept was performed by following at-line the relative concentration of a secreted protein produced by transient transfection of mammalian cells in a bioreactor. Our results suggest that our approach can be readily applied to the at-line determination of both protein concentration and bioactivity. Our experimental setup and strategy can thus satisfy the needs related to the development of novel bioprocess control protocols in the context of the new process analytical technology that arises in the biopharmaceutical industry.
Publication date
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In
  • Biotechnology and Bioengineering 100, no. May 1 (19 November 2007): 184–188.
NoteDA - 20080402IS - 1097-0290 (Electronic)LA - engPT - Journal ArticlePT - Research Support, Non-U.S. Gov'tRN - 0 (Recombinant Proteins)SB - IM
LanguageEnglish
Peer reviewedYes
NRC number47823
NRCC 47823
NPARC number12400992
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Record identifierb99769d5-7e41-4be6-af86-fc06a4292628
Record created2009-10-02
Record modified2020-05-10
Date modified: