Identification of pectenotoxin-11 as 34S-hydroxypectenotoxin-2, a new pectenotoxin analogue in the toxic donoflagellate Dinophysis acuta from New Zealand

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1021/tx050240y
Author	Search for: Suzuki, Toshiyuki¹; Search for: Walter, John A.²; Search for: LeBlanc, Patricia²; Search for: MacKinnon, Shawna³; Search for: Miles, Christopher O.; Search for: Wilkins, Alistair L.; Search for: Munday, Rex; Search for: Beuzenberg, Veronica; Search for: MacKenzie, A. Lincoln; Search for: Jensen, Dwayne J.; Search for: Cooney, Janine M.; Search for: Quilliam, Michael A.²
Affiliation	National Research Council Canada National Research Council Canada. Measurement Science and Standards National Research Council Canada. Aquatic and Crop Resource Development
Format	Text, Article
Subject	biological poisons; coasts; diarrhea; dinoflagellates; enzymes; hepatopancreas; magnetic resonance spectroscopy; marine molluscs; mass spectroscopy; mortality causes; phytoplankton; public health; toxicants; toxicity; toxicity tests; ultraviolet radiation; pectenotoxins; Perna canaliculus; Dinophysis acuta
Abstract	A new pectenotoxin, which has been named pectenotoxin-11 (PTX11), was isolated from the dinoflagellate Dinophysis acuta collected from the west coast of New Zealand. The structure of PTX11 was determined as 34S-hydroxypectenotoxin-2 by tandem mass spectrometry and UV and NMR spectroscopy. PTX11 appears to be only the third pectenotoxin identified as a natural biosynthetic product from algae after pectenotoxin-2 and pectenotoxin-12. The LD50 of PTX11 determined by mouse intraperitoneal injection was 244 g/kg. The LDmin of PTX11 in these experiments was 250 g/kg. No signs of toxicity were recorded in mice following an oral dose of PTX11 at 5000 g/kg. No diarrhea was observed in any of the animals administered with the test substance by either route of administration. Unlike pectenotoxin-2 (PTX2), PTX11 was not readily hydrolyzed to its corresponding seco acid by enzymes from homogenized green-lipped mussel (Perna canaliculus) hepatopancreas.
Publication date	2007-08-01
Terms of use	Copyright © 2006 American Chemical Society
In	Chemical Research in Toxicology 19, no. 2 (1 August 2007): 310–318.
Language	English
Peer reviewed	Yes
NRC number	NRCC 42555 1628
NPARC number	3538365
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Record identifier	b4b1270b-03a4-4c00-8dc6-86c38a7e9d52
Record created	2009-03-01
Record modified	2020-05-10

Date modified:: 2026-02-14