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Improved resolution of O-methylalditol acetates by gas-liquid chromatography on capillary columns with blended coatings of OV-17 and OV-225

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0008-6215(00)81077-0
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
Analysis of polysaccharides using the methylation technique has been aided by characterization and quantitative determination of derived O-methylalditol acetates by gas-liquid chromatography-mass spectrometry. Recent refinements in the technique include the use of capillary columns, which often results in better resolution of peaks. Recently, with the objective of analyzing microbial polysaccharides, the chromatographic behavior of O-methylalditol acetates arising from units ofgalactose, glucose, and mannose was examined on capillary columns of OV-17 and OV-225. In paralle1 and separate experiments with each column, it was found that several derivatives emerged in a different order depending on the column used. For example, the four tri-O-methylglucitol acetates that can be formed from glucopyranosyl units can be partly resolved on packed and capillary columns of OV-17, the 2,3,4 and 2,3,6
Publication date
PublisherElsevier
In
  • Carbohydrate Research 106, no. 2 (16 August 1982): 235–238.
LanguageEnglish
Peer reviewedYes
IdentifierS0008621500810770
NRCC-20133
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Record identifierb4aead6e-d1ac-4f55-a5a8-95ac6fba18d3
Record created2023-11-29
Record modified2023-11-29
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