| DOI | Resolve DOI: https://doi.org/10.1007/978-1-4939-8730-6_1 |
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| Author | Search for: L’Abbé, Denis1; Search for: Bisson, Louis1; Search for: Gervais, Christian1; Search for: Grazzini, Eric1; Search for: Durocher, Yves1 |
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| Affiliation | - National Research Council Canada. Human Health Therapeutics
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| Format | Text, Book Chapter |
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| Subject | transfection; 293-6E; polyethylenimine; deacylated PEI; recombinant protein; suspension culture; IMAC; protein-A; antibody; serum-free medium |
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| Abstract | Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram quantities of r-proteins can be typically obtained within less than 10 days following transfection. In this chapter, we describe a simple and robust transfection process of suspension-growing human embryo kidney 293 cells using two commercially available serum-free media and polyethylenimine as the transfection reagent. This chapter provides examples for the production and purification of a his-tagged recombinant protein and two monoclonal antibodies. |
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| Publication date | 2018-09-22 |
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| Publisher | Springer |
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| In | |
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| Series | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | b16e8413-e48e-4c23-93dd-71712cdffc2a |
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| Record created | 2019-05-03 |
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| Record modified | 2020-06-18 |
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