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Expression of abundant mRNAs during somatic embryogenesis of white spruce [Picea glauca (Moench) Voss]

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00195740
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectabscisic acid; embryo abundant gene; gene expression; polyethylene glycol; Picea; somatic embryogenesis
Abstract
Embryogenic tissues of white spruce [Picea glauca (Moench) Voss] remain in an early developmental stage while cultured on 2,4-dichlorophenoxyacetic acid and N6-benzyladenine, but develop to cotyledonary embryos when these phytohormones are replaced by abscisic acid. Twenty-eight cDNAs were isolated from cotyledonary embryos by differential screening against immature embryo and non-embryonic tissues. Temporal expression patterns of these cDNAs during ABA-stimulated somatic embryo development were observed. This showed that clones could be allocated to various groups, including embryo-abundant, embryo-maturation-induced, and those whose expression was modulated during embryo development, germination or in non-embryogenic tissues. Expression corresponding to these cDNA clones showed that there were various responses to exogenous ABA or polyethylene glycol during a period of 48 h. Analyses of DNA and predicted amino acid sequence revealed that 12 of 28 cDNA clones had no known homologues, while others were predicted to encode different late-embryogenesis-abundant proteins, early methionine-labelled proteins, storage proteins, heat-shock proteins, glycine-rich cell wall protein, metallothionein-like protein and some other metabolic enzymes.
Publication date
PublisherSpringer Nature
In
  • Planta 199, no. 3 (July 1996).
LanguageEnglish
Peer reviewedYes
IdentifierBF00195740
NRCC-38929
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Record created2023-11-02
Record modified2023-11-02
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