Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.jbiotec.2017.06.009
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1
Name affiliation
  1. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
Journal titleJournal of Biotechnology
ISSN0168-1656
1873-4863
Volume255
Pages16–27
SubjectCHO; cell pools; inducible expression system; methionine sulfoximine; polyethylenimine; recombinant proteins
Abstract
To rapidly produce large amounts of recombinant proteins, the generation of stable Chinese Hamster Ovary (CHO) cell pools represents a useful alternative to large-scale transient gene expression (TGE). We have developed a cell line (CHOBRI/rcTA) allowing the inducible expression of recombinant proteins, based on the cumate gene switch. After the identification of optimal plasmid DNA topology (supercoiled vs linearized plasmid) for PEIpro™ mediated transfection and of optimal conditions for methionine sulfoximine (MSX) selection, we were able to generate CHOBRI/rcTA pools producing high levels of recombinant proteins. Volumetric productivities of up to 900mg/L were reproducibly achieved for a Fc fusion protein and up to 350mg/L for an antibody after 14days post-induction in non-optimized fed-batch cultures. In addition, we show that CHO pool volumetric productivities are not affected by a freeze-thaw cycle or following maintenance in culture for over one month in the presence of MSX. Finally, we demonstrate that volumetric protein production with the CR5 cumate-inducible promoter is three- to four-fold higher than with the human CMV or hybrid EF1α-HTLV constitutive promoters. These results suggest that the cumate-inducible CHOBRI/rcTA stable pool platform is a powerful and robust system for the rapid production of gram amounts of recombinant proteins.
Publication date
PublisherElsevier
LanguageEnglish
Peer reviewedYes
NPARC number23002175
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Record identifierac970221-3075-4921-9c1d-fb59b097f911
Record created2017-08-29
Record modified2020-03-16
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