National Research Council of Canada. Measurement Science and Standards
National Research Council of Canada. Aquatic and Crop Resource Development
National Research Council of Canada. Energy, Mining and Environment
Dual excitation wavelength fluorometry was used for real-time monitoring and diagnosis of the growth of the microalgae Scenedesmus AMDD in a 300-L continuous photobioreactor (PBR). Emission spectra were acquired at 1-min intervals using excitation lights at 365 and 540 nm. Real-time dry weight estimations were achieved using linear regression with the chlorophyll peak, while protein estimations required a more complex Principal Component Regression model, which takes advantage of the full emission spectrum. The resulting regression coefficients were 0.95 and 0.80, respectively. Furthermore, the spectra were analyzed using multivariate curve resolution technique. The proposed approach for fluorescence-based, real-time measurements of key algae cultivation parameters and culture state diagnosis was successfully demonstrated in a 42-day PBR validation test.