| DOI | Resolve DOI: https://doi.org/10.1088/0960-1317/13/4/324 |
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| Author | Search for: Furdui, V.; Search for: Kariuki, J.; Search for: Harrison, D1 |
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| Affiliation | - National Research Council Canada. National Institute for Nanotechnology
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| Format | Text, Article |
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| Abstract | An integrated system for immunomagnetic separation of rare cells from blood is presented. A micromachined device was fabricated by bonding silicon die with etched structures to a glass cover plate on which electrodes are defined. Electrolytic generation of gas from 0.50 M KNO3 (aqueous) provided pumping actuation for a device that performed the capture and purification of rare cells spiked into a 7.5 ul reconstituted blood sample. The system consisted of two pumps, a sample and a wash buffer meander reservoir, and a main channel for magnetic field trapping of rare cells captured by antibody-coated magnetic beads. A maximum pumping rate of 1.4 +- 0.1 ul min[?]1 was obtained at a current of 180 uA, and the maximum blood sample volume delivered to the capture bed was 6.5-7 ul. The trapped cells could be washed with the buffer from the second pump and then delivered to the exit port of the chip after removing the magnetic field. |
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| Publication date | 2003 |
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| In | |
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| NPARC number | 12339031 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a9f78cb8-af9f-478c-a4d2-b0c8a2f7bca5 |
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| Record created | 2009-09-11 |
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| Record modified | 2020-04-02 |
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