| Author | Search for: Cox, A.; Search for: Li, J.; Search for: Brisson, J.; Search for: Moxon, E.; Search for: Richards, J. |
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| Format | Text, Article |
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| Subject | antibody; Canada; core oligosaccharide; group-B; interaction; Lipopolysaccharide; LPS; magnetic; magnetic-resonance; mass spectrometry; mass-spectrometry; monoclonal-antibodies; monoclonal-antibody; Neisseria; Neisseria meningitidis; NMR; nuclear; nuclear magnetic resonance; nuclear-magnetic-resonance; oligosaccharide; residues; resonance; specificity; spectroscopy; strain; structural analysis; structure |
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| Abstract | The structure of the phase-variable lipopolysaccharide (LPS) from the group B Neisseria meningitidis strain BZ157 galE was elucidated. The structural basis for the LPS's variation in reactivity with a monoclonal antibody (MAb) B5 that has specificity for the presence of phosphoethanolamine (PEtn) at the 3-position of the distal heptose residue (HepII) was established. The structure of the O-deacylated LPS was deduced by a combination of monosaccharide analyses, nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. These analyses revealed the presence of a novel inner core oligosaccharide (OS) structure in the MAb B5 reactive (B5+) LPS that contained two PEtn residues simultaneously substituting the 3- and 6-positions of the HepII residue. The determination of this structure has identified a further degree of variability within the inner core OS of meningococcal LPS that could contribute to the interaction of meningococcal strains with their host |
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| Publication date | 2002-09-09 |
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| In | |
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| Language | English |
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| NRC number | COX2002A |
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| NPARC number | 9388250 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a89c73e2-e620-4676-a397-3ac3b563f430 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-03-30 |
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