| Download | - View author's version: Higher-order structure of barley chromosomes observed by electron tomography (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1016/j.micron.2022.103328 |
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| Author | Search for: Hayashida, Misa1; Search for: Sartsanga, Channarong; Search for: Phengchat, Rinyaporn; Search for: Malac, Marek1; Search for: Harada, Ken; Search for: Akashi, Tetsuya; Search for: Fukui, Kiichi; Search for: Ohmido, Nobuko |
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| Affiliation | - National Research Council Canada. Nanotechnology
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| Funder | Search for: Japan Science and Technology Agency; Search for: Mitsubishi Foundation |
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| Format | Text, Article |
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| Subject | electron tomography; barley chromosomes; TEM |
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| Abstract | The higher order structure of the metaphase chromosome has been an enigma for over a century and several different models have been presented based on results obtained by a variety of techniques. Some disagreements in the results between methods have possibly arisen from artifacts caused during sample preparation such as staining and dehydration. Therefore, we treated barley chromosomes with ionic liquid to minimize the effects of dehydration. We also observed chromosomes on a film with holes to keep pristine chromosome structure from being flattened as seen when placed on a continuous support film. A chromosome placed over a hole in a thin carbon film was mounted on a tomography holder, and its structure was observed in three dimensions (3D) using electron tomography. We found that there are periodic structures with 300–400 nm pitch along the axis in barley chromosomes. The pitch sizes are larger than those observed in human chromosomes. |
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| Publication date | 2022-07-14 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S096843282200124X |
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| NRC number | NRC-NANO-231 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a5e483d6-5618-4fa1-ad50-eba8b74f3944 |
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| Record created | 2023-02-03 |
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| Record modified | 2023-02-07 |
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