| Download | - View supplementary information part 1: Cell envelope proteomics of mycobacteria (XLSX, 422 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/acs.jproteome.0c00650 |
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| Author | Search for: Hermann, Clemens; Search for: Karamchand, Leshern1; Search for: Blackburn, Jonathan M.ORCID identifier: https://orcid.org/0000-0001-8988-9595; Search for: Soares, Nelson C.ORCID identifier: https://orcid.org/0000-0003-2331-8532 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
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| Format | Text, Article |
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| Subject | cell wall proteomics; cell envelope; mass spectrometry; mycobacteria; tuberculosis; proteomics |
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| Abstract | The World Health Organization (WHO) estimates that Mycobacterium tuberculosis, the most pathogenic mycobacterium species to humans, has infected up to a quarter of the world’s population, with the occurrence of multidrug-resistant strains on the rise. Research into the detailed composition of the cell envelope proteome in mycobacteria over the last 20 years has formed a key part of the efforts to understand host–pathogen interactions and to control the current tuberculosis epidemic. This is due to the great importance of the cell envelope proteome during infection and during the development of antibiotic resistance as well as the search of surface-exposed proteins that could be targeted by therapeutics and vaccines. A variety of experimental approaches and mycobacterial species have been used in proteomic studies thus far. Here we provide for the first time an extensive summary of the different approaches to isolate the mycobacterial cell envelope, highlight some of the limitations of the studies performed thus far, and comment on how the recent advances in membrane proteomics in other fields might be translated into the field of mycobacteria to provide deeper coverage. |
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| Publication date | 2020-11-03 |
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| Publisher | American Chemical Society |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-NANO-189 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a1149c3c-6743-4d55-a12e-a6419da0a374 |
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| Record created | 2021-10-07 |
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| Record modified | 2021-10-07 |
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