Alkaloid production in Catharanthus roseus cell cultures: XII. biosynthetic capacity of callus from original explants and regenerated shoots

DOI	Resolve DOI: https://doi.org/10.1007/BF00269182
Author	Search for: Constabel, F.¹; Search for: Gaudet-LaPrairie, P.¹; Search for: Kurz, W. G. W.¹; Search for: Kutney, J. P.
Affiliation	National Research Council of Canada. Prairie Regional Laboratory
Format	Text, Article
Subject	cell culture; alkaloid; nutrient medium; zeatin; shoot formation
Abstract	Callus derived from hypocotyls of periwinkle, Catharanthus roseus, responded to culture on nutrient media supplementedwith IAA, BA, and zeatin with shoot formation at low frequencies. However, shoot regenerating callus could be very successfully propagated and subcultured. Alkaloid profiles of callus derived from the original explants (hypocotyls) as well as callus derived from regenerated shoots were almost identical. Subcultures of old callus (initiated in 1978) failed completely to grow shoots. In programs for long-term preservation of alkaloid producing cell lines by regeneration and storage of shoots, selection for ability to form shoots would have to precede selection for alkaloid production.
Publication date	1982-06
Publisher	Springer Nature
In	Plant Cell Reports 1, no. 4 (June 1982): 139–142.
Language	English
Peer reviewed	Yes
Identifier	BF00269182 NRCC-20087
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Record identifier	9ee380c5-cce5-47e0-9ae7-894368b5e5aa
Record created	2023-11-16
Record modified	2023-11-16