| DOI | Resolve DOI: https://doi.org/10.1016/j.bbrc.2014.02.064 |
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| Author | Search for: Chakravarthy, B.1; Search for: Ito, S.1; Search for: Atkinson, T.1; Search for: Gaudet, C.1; Search for: Ménard, M.1; Search for: Brown, L.1; Search for: Whitfield, J.1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | amyloid beta protein; amyloid beta protein[1-42]; amyloid beta protein[25-35]; oligomer; synthetic peptide; Alzheimer disease; animal experiment; animal model; animal tissue; apoptosis; brain; brain cortex; brain tissue; hippocampus; human tissue; immunohistology; microinjection; mouse; neuroblast; nucleotide sequence; protein aggregation; protein targeting; transgenic mouse; Mus musculus; AD transgenic mice; Alzheimer's disease; Amyloid binding peptide; Aβ(1-42) oligomers; Human AD brains; PCM-1 protein; Aged; Aged, 80 and over; Alzheimer Disease; Amino Acid Sequence; Amyloid beta-Peptides; Autoantigens; Brain; Cell Cycle Proteins; Cell Line; Male; Mice; Mice, Transgenic; Middle Aged; Molecular Sequence Data; Peptide Fragments; Peptides; Protein Binding |
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| Abstract | The synthetic ~5kDa ABP (amyloid-ß binding peptide) consists of a region of the 228kDa human pericentrioloar material-1 (PCM-1) protein that selectively and avidly binds in vitro Aβ1-42 oligomers, believed to be key co-drivers of Alzheimer's disease (AD), but not monomers (Chakravarthy et al., (2013) [3]). ABP also prevents Aß1-42 from triggering the apoptotic death of cultured human SHSY5Y neuroblasts, likely by sequestering Aß oligomers, suggesting that it might be a potential AD therapeutic. Here we support this possibility by showing that ABP also recognizes and binds Aβ1-42 aggregates in sections of cortices and hippocampi from brains of AD transgenic mice and human AD patients. More importantly, ABP targets Aβ1-42 aggregates when microinjected into the hippocampi of the brains of live AD transgenic mice. © 2014. |
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| Publication date | 2014 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21272242 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 99784d6c-3fd9-4e99-9772-3d13f62a4021 |
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| Record created | 2014-07-23 |
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| Record modified | 2020-04-22 |
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