| DOI | Resolve DOI: https://doi.org/10.1038/sj.jim.2900773 |
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| Author | Search for: Chung, B. H.; Search for: Choi, Y. J.; Search for: Yoon, S. H.; Search for: Lee, S. Y.; Search for: Lee, Y. I. |
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| Format | Text, Article |
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| Abstract | Fed-batch cultures were carried out to overproduce human insulin-like growth factor I (IGF-I) in Escherichia coli. The effects of carbon sources (glucose or glycerol) and induction time on cell growth and IGF-I production were investigated in more detail. Glycerol was a better carbon source than glucose for IGF-I production in fed-batch culture. Induction at the mid-exponential phase with glycerol as a carbon source in the pH-stat fed-batch culture was optimal for IGF-I production. Under this condition, 2.8 g L[SUP-1] of fusion IGF-I was produced as inclusion bodies. We have also developed downstream processing for preparative scale purification of IGF-I from the fusion protein produced by the fed-batch culture using glycerol as a carbon source. After the fusion protein expressed was solubilized in 8 M urea and cleaved with hydroxylamine, the released IGF-I was purified by cation exchange chromatography, refolding and preparative scale reverse phase HPLC (rp-HPLC) to give recombinant IGF-I of >98% purity. The biological activities of the purified IGF-I were measured and found to be identical to those of commercial IGF-I. |
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| Publication date | 2000-02-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC publication | This is a non-NRC publication"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC. |
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| NPARC number | 23001828 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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| Record identifier | 983f89c3-6b7b-46b7-a7f0-6556540cf123 |
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| Record created | 2017-04-13 |
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| Record modified | 2020-03-26 |
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