| Download | - View final version: Limitations and optimizations of cellular lineages tracking (PDF, 4.8 MiB)
- View supplementary information: Limitations and optimizations of cellular lineages tracking (PDF, 1.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1371/journal.pcbi.1012880 |
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| Author | Search for: Leibovich, Nava1ORCID identifier: https://orcid.org/0009-0000-9182-3640; Search for: Goyal, Sidhartha |
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| Editor | Search for: Uhlmann, Virginie |
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| Affiliation | - National Research Council of Canada. Digital Technologies
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| Funder | Search for: Medicine by Design (MbD); Search for: Natural Sciences and Engineering Research Council of Canada (NSERC) |
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| Format | Text, Article |
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| Subject | cloning; RNA sequencing; species diversity; gene pool; vector-borne diseases; experimental design; probability distribution; viral vector techniques |
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| Abstract | Tracking cellular lineages using genetic barcodes provides insights across biology and has become an important tool. However, barcoding strategies remain ad hoc. We show that elevating barcode insertion probability and thus increasing the average number of barcodes within the cells, adds to the number of traceable lineages but may decrease the accuracy of lineages inference due to reading errors. We establish the trade-off between accuracy in tracing lineages and the total number of traceable lineages, and find optimal experimental parameters under limited resources concerning the populations size of tracked cells and barcode pool complexity. |
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| Publication date | 2025-04-14 |
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| Publisher | Public Library of Science (PLOS) |
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| Licence | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 94d06d30-494d-43af-a360-2276803a1476 |
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| Record created | 2025-05-08 |
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| Record modified | 2025-06-03 |
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