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Expression systems for therapeutic glycoprotein production

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.copbio.2009.10.008
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Affiliation
  1. National Research Council Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectactivity; apoptosis; baculovirus; bcl-2; biotechnology; bioreactors; blood; caspase 3; cell culture; cell line; cell; chickens; DNA; DNA methylation; endoplasmic reticulum; enzymes; enzymology; epidermal growth factor; galactose; genes; genetic engineering; genome; glycoproteins; glycosylation; heat; HIV-1; IGG; immunoglobulin g; insect cell; lectins; metabolism; microbiology; peptide; Pichia; plant; protein; protein production; rabbit; recombinant proteins; RNA; saccharomyces cerevisiae; secretion; sodium; stress; synthesis; T-lymphocytes; temperature; transfection; tyrosine; yeast
Abstract
There are slightly over 165 recombinant pharmaceuticals currently approved for human use. Another 500 protein candidates are in preclinical and clinical development, about 70% of these being glycosylated proteins. The need for expression systems allowing the efficient manufacturing of high quality glycoproteins is thus becoming imperative. Recent developments with CHO cells, the predominant mammalian expression system, have focused on either increasing cell specific productivity or prolonging the life span of cells in culture that translates to high integrated viable cell densities. These two factors have allowed volumetric productivities in excess of 5 g/L under conditions of controlled nutrient feeding. In addition to glycoengineering strategies, which are offering considerable advantage in producing proteins with enhanced therapeutic properties, several alternative expression systems are being developed for their manufacture, each with their advantages and limitations.
Publication date
In
  • Current Opinion in Biotechnology 20, no. 6 (4 November 2009): 700–707.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 52743
NPARC number14022219
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Record identifier94328d3a-c8d6-4175-80a0-ac5e9c57c310
Record created2010-06-02
Record modified2020-04-16
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