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The SCFᶜᵒᴵ¹ ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1105/tpc.003368
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Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
Abstract
Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase complexes, which we have designated SCFᶜᵒᴵ¹. COI1ₑ₂₂ₐ, a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the SCFᶜᵒᴵ¹ complexes and results in loss of the JA response. AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression. Furthermore, we show that the AtCUL1 component of SCFᶜᵒᴵ¹ complexes is modified in planta, where mutations in AXR1 decrease the abundance of the modified AtCUL1 of SCFᶜᵒᴵ¹ and lead to a reduction in JA response. Finally, we demonstrate that the axr1 and coi1 mutations display a synergistic genetic interaction in the double mutant. These results suggest that the COI1-mediated JA response is dependent on the SCFᶜᵒᴵ¹ complexes in Arabidopsis and that the AXR1-dependent modification of the AtCUL1 subunit of SCFᶜᵒᴵ¹ complexes is important for JA signaling.
Publication date
PublisherOxford Academic
American Society of Plant Biologists
In
  • Plant Cell 14, no. 8 (1 August 2002): 1919–1935.
LanguageEnglish
Peer reviewedYes
NPARC number13052707
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Record identifier8c387742-adbb-4bc7-8014-5d55c9f4f5b8
Record created2009-11-25
Record modified2023-04-14
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