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Isolation and characterization of two antimicrobial peptides from haemocytes of the American lobster Homarus americanus

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.fsi.2008.04.005
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Affiliation
  1. National Research Council of Canada. NRC Institute for Marine Biosciences
FormatText, Article
Abstract
Two antimicrobial peptides from haemocytes of the American lobster, Homarus americanus H. Milne Edwards 1837, were isolated and partially characterised – the first such description for this species. CAP-2, an approximately 12 kDa peptide, contained amino acid sequences corresponding to the predicted sequence for Hoa-crustin. Crustins are whey acidic protein (WAP) domain - containing peptides isolated from crustacean haemocytes. CAP-2 did not have any activity towards the Gram positive coccus Aerococcus viridans unlike carcinin, a crustin from Carcinus maenas haemocytes, which may partially explain the lobster's susceptibility to this bacterium. A second peptide, CAP-1, was a multimer composed of 4–6 kDa subunits with similarities to amphibian temporins. CAP-1 may represent a novel group of antimicrobial peptides for marine invertebrates and has been tentatively named ‘homarin’. Homarin had bacteriostatic activity against some Gram negative bacteria and both protozoastatic and protozoacidal activity against two cultured scuticociliate parasites Mesanophrys chesapeakensis and Anophryoides haemophila, the latter a significant pathogen of H. americanus.
Publication date
In
  • Fish & Shellfish Immunology 25 (July 2008): 181–184.
LanguageEnglish
Peer reviewedYes
NRC numberNRCC 42785
1749
NPARC number3538522
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Record identifier853f6b0b-e808-499d-be8e-ca4c2b1c0a8e
Record created2009-03-01
Record modified2020-04-15
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