| Download | - View final version: Microfluidic integration of magnetically functionalized microwires for flow cytometry protein quantification (PDF, 2.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.3390/ma18020215 |
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| Author | Search for: Clime, Liviu1ORCID identifier: https://orcid.org/0000-0002-1786-0803; Search for: Pavel, CatalinORCID identifier: https://orcid.org/0009-0008-6069-0425; Search for: Malic, Lidija1ORCID identifier: https://orcid.org/0000-0001-8594-9191; Search for: Nassif, Christina1; Search for: Geissler, Matthias1ORCID identifier: https://orcid.org/0000-0002-8838-5528; Search for: Lupu, Nicoleta; Search for: Óvári, Tibor-AdrianORCID identifier: https://orcid.org/0000-0003-3074-7749; Search for: Poncelet, Lucas1ORCID identifier: https://orcid.org/0000-0002-1376-9367; Search for: Veilleux, Gaétan1; Search for: Moslemi, Elham1; Search for: Hernández-Castro, Javier Alejandro1ORCID identifier: https://orcid.org/0000-0002-1084-4392; Search for: Sinnett, DanielORCID identifier: https://orcid.org/0000-0003-3625-6676; Search for: Che, Diping; Search for: Veres, Teodor1ORCID identifier: https://orcid.org/0000-0002-9594-8042 |
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| Affiliation | - National Research Council Canada. Medical Devices
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| Format | Text, Article |
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| Subject | microfluidics; protein quantification; magnetic microwires; magnetic nanoparticles; anocrystalline magnets; flow cytometry |
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| Abstract | A novel approach to protein quantification utilizing a microfluidic platform activated by a magnetic assembly of functionalized magnetic beads around soft magnetic capture centers is presented. Functionalized magnetic beads, known for their high surface area and facile manipulation under external magnetic fields, are injected inside microfluidic channels and immobilized magnetically on the surface of glass-coated soft magnetic microwires placed along the symmetry axis of these channels. A fluorescent (Cy5) immunomagnetic sandwich ELISA is then performed by sequentially flowing the sample and all necessary reagents in the microfluidic channels. Direct protein quantification is performed by magnetically releasing the beads from the microwire and evaluating their fluorescence intensity with the help of a miniature (microfluidic-based) flow cytometer. Measurements of ICAM-1 protein concentration in human blood plasma samples confirm the feasibility of the approach through extensive performance benchmarking. The automation and multiplexing capabilities of the proposed platform further demonstrate its potential for protein quantification in point-of-care settings using microfluidics and miniature flow cytometry instruments. |
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| Publication date | 2025-01-07 |
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| Publisher | MDPI |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 82154c45-c159-4e81-830e-fbfa8bff3cb1 |
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| Record created | 2025-06-19 |
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| Record modified | 2025-06-19 |
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