| DOI | Resolve DOI: https://doi.org/10.1002/cbic.200700116 |
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| Author | Search for: Hui, Joseph P. M.1; Search for: Yang, Jie; Search for: Thorson, Jon S.; Search for: Soo, Evelyn C.2 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
- National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | analytical methods; bioorganic chemistry; electrophoresis; glycorandomization; mass spectrometry; sugar phosphates |
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| Abstract | A highly selective method employing capillary electrophoresis and electrospray mass spectrometry (CE-ESMS) with precursor ion scanning for fragment ions characteristic of phosphate-linked sugars was developed for the determination of unnatural sugar phosphates generated in vivo, as part of a natural product glycorandomization study. Cell lysates from an engineered E. coli host were probed for natural and unnatural sugar phosphates resulting from in vivo galactokinase (GalK) bioconversions, and tandem mass spectrometry experiments were performed to confirm the identities of the sugar phosphates. Among the 22 cell lysates that were studied, 13 were found to contain the expected natural and unnatural sugar phosphates. This was in agreement with the GalK in vitro conversion yields, in which an in vitro yield of 15 % coincided with a lack of observable in vivo bioconversion. In addition, the CE-ESMS and precursor ion scanning method was capable of separating sugar phosphate regioisomers such as hexose-6-phosphate and hexose-1-phosphate. |
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| Publication date | 2007-07-09 |
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| Terms of use | - Copyright © 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 42705
1242 |
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| NPARC number | 3538056 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 80a469eb-9cbb-43ac-b86d-1307b1370362 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-05-10 |
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