Delphinidin, a dietary anthocyanidin, inhibits vascular endothelial growth factor receptor-2 phosphorylation

From National Research Council Canada

Download
  1. (PDF, 483 KB)
DOIResolve DOI: https://doi.org/10.1093/carcin/bgi279
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: 1; Search for: ; Search for: ; Search for: ; Search for:
Name affiliation
  1. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
Journal titleCarcinogenesis
ISSN0143-3334
1460-2180
Volume27
Issue5
Pages989–996
SubjectbFGF; basic fibroblast growth factor; Cy; cyanidin; Dp; delphinidin; Dp 3-glu; delphinidin 3- O -beta-glucopyranoside; EC; endothelial cell; ERK; extracellular-signal-regulated kinase; HUVEC; human umbilical vein endothelial cell; Mv; malvidin; Pg; pelargonidin; Pt; petunidin; STS; staurosporine; VEGF; vascular endothelial growth factor; VEGFR-2; vascular endothelial growth factor receptor-2
Abstract
Epidemiological studies have shown that a diet rich in fruits and vegetables has a beneficial preventive effect on cardiovascular diseases and cancer by mechanisms that have not yet been elucidated. In this work, we investigated the antiangiogenic activities of anthocyanidins, a class of polyphenols present at high levels in fruits. Among the tested anthocyanidins (cyanidin, delphinidin, malvidin, pelargonidin, peonidin and petunidin), delphinidin was the most potent angiogenic inhibitor. In vitro , low concentrations of delphinidin inhibited vascular endothelial growth factor (VEGF)-induced tyrosine phosphorylation of VEGF receptor (VEGFR)-2, leading to the inhibition of downstream signaling triggered by VEGFR-2. Inhibition of VEGFR-2 by delphinidin inhibited the VEGF-induced activation of ERK-1/2 signaling and the chemotactic motility of human EC as well as their differentiation into capillary-like tubular structures in Matrigel and within fibrin gels. In vivo , delphinidin was able to suppress basic fibroblast growth factor-induced vessel formation in the mouse Matrigel plug assay. The identification of delphinidin as a naturally occurring inhibitor of VEGF receptors suggests that this molecule possesses important antiangiogenic properties that may be helpful for the prevention and treatment of cancer.
Publication date
PublisherOxford University Press
Peer reviewedYes
NRC numberNRC-BRI-47792
NPARC number3538861
Export citationExport as RIS
Report a correctionReport a correction
Record identifier7f3b2a42-474b-4a3a-bec6-285f5ec2501f
Record created2009-03-01
Record modified2020-05-27
Report a problem on this page
Date modified: