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Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticles

From National Research Council Canada

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DOIResolve DOI: https://doi.org/10.1016/j.xpro.2025.103859
AuthorSearch for: ORCID identifier: https://orcid.org/0000-0003-4492-4910; Search for: 1ORCID identifier: https://orcid.org/0000-0001-8594-9191; Search for: 1; Search for: 1; Search for:
Affiliation
  1. National Research Council Canada. Medical Devices
FormatText, Article
Subjectbiotechnology and bioengineering; high-throughput screening; molecular biology; molecular/chemical probes; sequencing
Abstract
The generation of combinatorial oligonucleotide libraries is desirable for applications such as DNA aptamers, data storage, DNA origami, or synthetic genomes, but conventional libraries present challenges in detection and analysis. Synthesis of unique oligonucleotide sequences on magnetic nanoparticles would enhance the ability to manipulate, recover, and detect them. Here, we present a protocol for generating a scalable combinatorial oligonucleotide library on magnetic nanoparticles using split-and-pool synthesis. We then describe the process for preparing the library for conventional and next-generation sequencing (NGS) DNA sequencing.
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PublisherElsevier
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  • STAR Protocols 6, no. 2, 103859 (30 May 2025).
LanguageEnglish
Peer reviewedYes
IdentifierS2666166725002655
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Record identifier7c8c6f5c-cfbf-467f-b02e-505571b8d156
Record created2025-07-31
Record modified2025-07-31
Date modified: