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Defining steric, electronic and conformational requirements of carrier-mediated uptake of abscisic acid in barley suspension culture cells

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/S0031-9422(97)00299-9
AuthorSearch for: ; Search for: 1; Search for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Plant Biotechnology Institute
FormatText, Article
SubjectHordeum vulgare; gramineae; barley suspension culture cells; abscisic acid uptake; inhibitors; carrier; abscisic acid
Abstract
The structural and conformational requirements of a carrier responsible for the saturable component of the uptake of the plant hormone (+)-abscisic acid in barley suspension culture cells have been probed through the use of a defined series of optically pure ABA analogues. Two analogues showed a tenfold increase over (+)-ABA in inhibiting the uptake of radiolabelled (+)-ABA. Results from different studies (molecular modelling, low temperature NMR spectroscopy and assays with sterically rigid analogues) have shown the likely conformation of ABA in the binding site of the carrier is that with the sidechain in an equatorial-like orientation. Structure/activity studies show that the C-1 carboxylic acid is essential for binding, the C-4 carbonyl is moderately important while the C-1′ hydroxyl group is not important for binding to the carrier.
Publication date
PublisherElsevier
In
  • Phytochemistry 46, no. 2 (September 1997): 215–222.
LanguageEnglish
Peer reviewedYes
IdentifierS0031942297002999
NRCC-40713
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Record identifier78a8f3bd-0264-4b7c-aacf-87c20c30120a
Record created2023-11-01
Record modified2023-11-01
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