Rapid microwave assisted esterification method for the analysis of poly-3-hydroxybutyrate in Alcaligenes latus by gas chromatography

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Name affiliation
  1. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
Journal titleJournal of Chromatography A
Volume1154
Issue1-2
Pages473–476
Subjectenvironmental
Abstract
In the present study, we used previous termmicrowavenext term energy instead of conventional heating to transform poly-3-hydroxybutyrate (PHB) into methyl 3-hydroxybutyrate (Me-3HB) in acidified methanol (H2SO4, 10%, v/v) mixture in less than 4 min at 10% previous termmicrowavenext term power. The previous termmicrowavenext term assisted method was then applied to analyze PHB produced by Alcaligenes latus. The PHB content in the biomass determined using previous termmicrowavenext term heating was comparable to the amount found by conventional heating. Moreover, the new esterification method was at least 50 times faster than the conventional method, affording a significant saving of time and energy.
Publication date
LanguageEnglish
NRC number49035
NPARC number3539443
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