| DOI | Resolve DOI: https://doi.org/10.1016/S0044-328X(79)80208-1 |
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| Author | Search for: Brar, D.S.1; Search for: Rambold, S.1; Search for: Gamborg, O.1; Search for: Constabel, F.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Subject | Sorghum bicolor; Zea mays; cell suspension culture; plant regeneration; polyploidization |
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| Abstract | A fast growing cell suspension culture of sorghum (Sorghum bicolor L. Moench) has been established. Out of 15 genetic strains, cv. GPR-168 responded most favorably to conditions for callus formation. The callus was friable and upon transfer to liquid medium produced cell suspension cultures. During 96 h of subculturing the cells showed mitotic indices of 1.0 to 6.5 %. Plant regeneration from callus derived from seedlings and immature embryos was achieved in two sorghum strains. Cytology of callus, cell suspension cultures, and regenerated plants indicated a strong prevalence of polyploidization.
The study also includes observations on a fast growing cell culture of corn. The culture had a doubling time of 48 h and during a 72 h period showed mitotic indices from 3.6 to 10.9 %. Protoplasts were readily isolated from the cell culture, but spontaneous fusion occurred extensively. On average 52 % of the isolated protoplasts were multinucleated. Attempts to eliminate the phenomenon were not successful. During subsequent culture some of the protoplasts regenerated cell walls and limited division occurred. |
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| Publication date | 1979-12 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0044328X79802081 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 75f26d25-7ed9-4a5b-b83a-5bc2c49b8cda |
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| Record created | 2023-07-25 |
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| Record modified | 2023-07-25 |
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