| DOI | Resolve DOI: https://doi.org/10.1016/j.vaccine.2010.03.029 |
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| Author | Search for: Le Ru, Audrey1; Search for: Jacob, Danielle1; Search for: Transfiguracion, Julia1; Search for: Ansorge, Sven1; Search for: Henry, Olivier; Search for: Kamen, Amine A.1 |
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| Affiliation | - National Research Council Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | acids; activity; biotechnology; adenovirus; apoptosis; bioreactor; cell culture; cell death; cell line; cell; centrifugation; chromatography; comparative study; gene expression; gene therapy; gene; glycosylation; HEK-293; HEK-293 cells; hemagglutinin; in vitro; influenza; influenza vaccine; influenza virus; insect cell; iodixanol; kidney; kinetics; metabolism; morphogenesis; neuraminidase; nucleic acids; oxygen; protein; protein production; purification; recombinant protein; recombinant proteins; RNA; scalable processes; serum-free medium; suspension culture; synthesis; therapy; transfection; vaccine production; virology; virulence; virus production; virus replication; vitro |
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| Abstract | Cell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10<sup>9</sup> IVP/mL demonstrating the scale-up potential of the process. Crown Copyright -¬ 2010 |
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| Publication date | 2010-07-05 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 52752 |
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| NPARC number | 16225341 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 708c5796-3c2c-4ca2-ae94-4437c91b1829 |
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| Record created | 2010-11-05 |
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| Record modified | 2020-04-17 |
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