| DOI | Resolve DOI: https://doi.org/10.1093/glycob/cwaa069 |
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| Author | Search for: St Michael, Frank1; Search for: Cairns, Chantelle M.1; Search for: Fleming, Perry1; Search for: Vinogradov, Evgeny V.1; Search for: Boyce, John D.; Search for: Harper, Marina; Search for: Cox, Andrew D.1ORCID identifier: https://orcid.org/0000-0002-8397-9271 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | capsular polysaccharides; Pasteurella multocida; NMR; serotyping; structure |
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| Abstract | We describe the structural characterization of the capsular polysaccharides (CPSs) of Pasteurella multocida serotypes B and E. CPS was isolated following organic solvent precipitation of the supernatant from flask grown cells. Structural analysis utilizing nuclear magnetic resonance spectroscopy enabled the determination of the CPS structures and revealed significant structural similarities between the two serotypes, but also provided an explanation for the serological distinction. This observation was extended by the development of polyclonal sera to the glycoconjugate of serotype B CPS that corroborated the structural likenesses and differences. Finally, identification of these structures enabled a more comprehensive interrogation of the genetic loci and prediction of roles for some of the encoded proteins in repeat unit biosynthesis. |
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| Publication date | 2020-07-28 |
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| Publisher | Oxford University Press |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 6bf31659-9830-497c-8c46-106881af04f9 |
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| Record created | 2023-05-02 |
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| Record modified | 2023-05-02 |
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