| DOI | Resolve DOI: https://doi.org/10.1016/j.bbrc.2008.11.036 |
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| Author | Search for: Atkinson, Trevor1; Search for: Whitfield, James1; Search for: Chakravarthy, Balu1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | 1-phosphatidylinositol 3-kinase; acid; animals; antagonists & inhibitors; Okadaic acid; IGF-1; bFGF; neuroprotection; oxygen-glucose deprivation; cortical neuron; brain; canada; cells,cultured; cerebral Cortex; cytology; cytoprotection; drug effects; enzyme; enzyme inhibitors; enzymology; glucose; inhibitor; inhibitors; metabolism; mitogen-activated protein kinase kinases; molecular; neurons; neuroprotective agents; okadaic acid; oxygen; pathway; pathways; peptide; pharmacology; phosphoric monoester hydrolases; phosphorylation; protection; protein; proteins; proto-oncogene proteins c-akt; rats; synthesis |
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| Abstract | The protein kinase-mediated actions of peptide growth factors such as IGF-1 and bFGF protect cultured neurons from being killed by the oxygen and glucose deprivations (OGD) that prevail in the ‘stroked brain’. Here, we show that neuroprotection by IGF-1 is mediated by PI-3K/Akt, whereas that of bFGF is mediated by MAPK. IGF-1 and bFGF together did not further increase protection suggesting a downstream convergence of their pathways. Since protein kinases mediated the protection, a phosphatase inhibitor such as okadaic acid (OA) might be as protective as the growth factors against OGD. Here, we show that OA is actually a much more effective protector. It increased the phosphorylation of both PI-3K/Akt and MAPK, and stimulated new protein synthesis. OA also acted independently of the CREB activation and FKHRL1 and GSK-3 inactivation which have been implicated in IGF-1 actions. |
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| Publication date | 2008-11-19 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 15329252 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 6a495f7b-9c39-42b9-8aaf-4fe4346fbb54 |
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| Record created | 2010-05-21 |
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| Record modified | 2020-04-15 |
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