| Author | Search for: Yan, F.; Search for: Gilbert, M.; Search for: Wakarchuk, Warren; Search for: Brisson, J.; Search for: Whitfield, Dennis |
|---|
| Format | Text, Article |
|---|
| Subject | Canada; Carbohydrate Conformation; chemical synthesis; chemistry; donor; ENZYME; enzymes; Glycosylation; Glycosyltransferases; LACTOSE; LINKAGES; metabolism; MOLECULE; NOE; Nuclear Magnetic Resonance,Biomolecular; oligosaccharide; Oligosaccharides; Polyethylene Glycols; Protein Binding; SHIGA-LIKE TOXIN; Shiga-Like Toxin I; Support,Non-U.S.Gov't; Synthesis; synthetic; transfer; TRISACCHARIDE; Trisaccharides |
|---|
| Abstract | [reaction: see text]. A trisaccharide donor containing a cis-Galpalpha(1-->4)Galp linkage was prepared using a synthetic strategy based on chemoenzymatic oligosaccharide synthesis on a soluble polymeric support. Significantly, only retaining glycosyltransferases gave complete reactions, whereas inverting enzymes showed little or no activity with poly(ethylene glycol) (MPEG)-bound lactose as an acceptor. The MPEG-attached trisaccharide was shown to bind to Verotoxin-1 by transfer NOE studies through the Galpalpha(1-->4)Galp portion of the molecule |
|---|
| Publication date | 2001-10-18 |
|---|
| In | |
|---|
| Language | English |
|---|
| NRC number | YAN2001A |
|---|
| NPARC number | 9360321 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 657615ac-cdaf-47a5-874d-3cd65e103d1e |
|---|
| Record created | 2009-07-10 |
|---|
| Record modified | 2020-03-27 |
|---|
