Fourier Transform Infrared (FTIR) studies of the lipid thermotropic mesomorphism of Acholeplasma laidlawii B

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1117/12.932159
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Name affiliation
  1. National Research Council of Canada
FormatText, Book chapter
Proceedings title1981 International Conference on Fourier Transform Infrared Spectroscopy
Series titleProceedings of SPIE
Conference1981 International Conference on Fourier Transform Infrared Spectroscopy, October 29, 1981, Hajime Sakai, Columbia
ISSN0277-786X
Volume289
Pages169–171
Abstract
The lipid components of the plasma membrane of the microorganism A. laidlawii B undergo a temperature induced phase transition. The biosynthetic incorporation of deuterated fatty acids into these membranes has allowed, via the C-D stretching vibrations, a detailed study of the lipid organization and dynamics, and the effect of the intrinsic membrane proteins on the transition. A number of fatty acids, of different chain lengths, have been incorporated biosynthetically in the presence or absence of avidin, a fatty acid synthesis inhibitor. In this manner the membranes are enriched solely in the added deuterated fatty acid up to high levels of homogeneity, providing an excellent system for the study of lipid-protein interactions. Detailed data regarding the lipid thermal behaviour will be presented and compared with results obtained by means of other techniques.
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PublisherSPIE
LanguageEnglish
Peer reviewedYes
NPARC number23001113
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