| DOI | Resolve DOI: https://doi.org/10.1194/jlr.D900013-JLR200 |
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| Author | Search for: Zhou, Ping1; Search for: Chandan, Vandana1; Search for: Liu, Xin1; Search for: Chan, Kenneth1; Search for: Altman, Eleonora1; Search for: Li, Jianjun1 |
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| Affiliation | - National Research Council Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Helicobacter pylori; acylation; microwave-assisted digestion; mass spectrometry; MALDI-TOF/TOF |
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| Abstract | The lipid A of Gram-negative bacteria plays a major role in the pathogenesis of bacterial infections. Lipid A diversity is observed both in the number and length of fatty acid side chains and in the presence of terminal phosphate residues and associated modifications. In this report, we describe a new sample preparation method based on microwave-assisted enzymatic digestion and detergent-free mild hydrolysis, in conjunction with a MALDI-time-of-flight (TOF)/TOF analysis, to determine the structures of lipid A from Helicobacter pylori . The total time for sample preparation and mass spectrometric analysis is within 2 h and applicable to profiling the lipid A structures from dried bacterial cells on as little as 1 µg. The reliability of the technique was further demonstrated through the analysis of the lipid A from bacterial cells of different H. pylori strains. The phosphorylation and acylation patterns of lipid A could be elucidated using material from a single colony. Furthermore, we found unusual heptaacyl lipid A species present in H. pylori mutant that have not been previously reported, although the abundance was relatively low. The present study provides the first characterization of the lipid A component from a single bacterial colony sample by mass spectrometry. |
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| Publication date | 2009-09 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 15329308 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 625f2850-6018-45c8-b3b8-1409129ce650 |
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| Record created | 2010-05-21 |
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| Record modified | 2020-04-16 |
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