| DOI | Resolve DOI: https://doi.org/10.1016/j.jnutbio.2015.05.014 |
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| Author | Search for: Schenkel, Laila C.; Search for: Sivanesan, Sugashan; Search for: Zhang, Junzeng1; Search for: Wuyts, Birgitte; Search for: Taylor, Adrian; Search for: Verbrugghe, Adronie; Search for: Bakovic, Marica |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | choline; fatty acid; homocysteine; phosphatidylethanolamine; sphingomyelin; triacylglycerol; adipocyte; animal experiment; diet therapy; enzyme deficiency; fatty liver; gene control; hypertriglyceridemia; lipid oxidation; liver function; male; mouse; obesity; phosphoethanolamine cytidylyltransferase 2 deficiency; phosphoethanolamine cytidylyltransferase 2 deficiency; supplementation; synthesis; triacylglycerol blood level; turnover time |
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| Abstract | Choline plays a critical role in systemic lipid metabolism and hepatic function. Here we conducted a series of experiments to investigate the effect of choline supplementation on metabolically altered Pcyt2+/- mice. In Pcyt2+/- mice, the membrane phosphatidylethanolamine (PE) turnover is reduced and the formation of fatty acids (FA) and triglycerides (TAG) increased, resulting in hypertriglyceridemia, liver steatosis and obesity. One month of choline supplementation reduced the incorporation of FA into TAG and facilitated TAG degradation in Pcyt2+/- adipocytes, plasma and liver. Choline particularly stimulated adipocyte and liver TAG lipolysis by specific lipases (ATGL, LPL and HSL) and inhibited TAG formation by DGAT1 and DGAT2. Choline also activated the liver AMPK and mitochondrial FA oxidation gene PPARα and reduced the FA synthesis genes SREBP1, SCD1 and FAS. Liver (HPLC) and plasma (tandem mass spectroscopy and 1H-NMR) metabolite profiling established that Pcyt2+/- mice have reduced membrane cholesterol/sphingomyelin ratio and the homocysteine/methionine cycle that were improved by choline supplementation. These data suggest that supplementary choline is beneficial for restoring FA and TAG homeostasis under conditions of obesity caused by impaired PE synthesis. |
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| Publication date | 2015-07-08 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21277378 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 60ea0b38-6b2b-4bb2-a259-4100ad4ca370 |
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| Record created | 2016-03-09 |
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| Record modified | 2020-04-22 |
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