The temperature dependences of the Raman spectra of aqueous dispersions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) were monitored at different but constant pressures between 1 and 1210 bar. The changes observed in these Raman spectra are discussed in terms of the effects of high pressure on the phase state and molecular structure of lipid bilayers. It is demonstrated that the temperature of the endothermic gel to liquid-crystal phase transition, as well as the temperature of the pretransition, increases linearly with increasing hydrostatic pressure. The dT,/dP values obtained from a wide range of pressures are 20.8 OC-kbar⁻¹ for DPPC and 20.1 OCmkbar⁻¹ for DMPC. The dTp/dP value for DPPC is 16.2 OCºkbar⁻¹. It is also shown that the volume change that occurs at the gel to liquid-crystal transition is not constant; i.e., dΔVm/dP decreases by 6.2% (DPPC) or 6.3% (DMPC) per kilobar pressure. The volutie change at the pretransition is also pressure dependent; the dΔVp/dP value of DPPC decreases by 4.7% per kilobar pressure.