| Author | Search for: Cox, A.; Search for: Masoud, H.; Search for: Thibault, P.; Search for: Brisson, J.; Search for: Van Der, Zwan; Search for: Perry, Malcolm; Search for: Richards, J. |
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| Format | Text, Article |
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| Subject | Canada; carbohydrate conformation; classification; core region; Haemophilus influenzae; isolation & purification; Lipopolysaccharides; LPS; magnetic resonance spectroscopy; mass spectrometry; methylation; molecular sequence data; NMR; NMR spectroscopy; oligosaccharides; phosphorylcholine; region; residues; series; spectrometry, mass, electrospray ionization; strain; structural analysis; structure; trisaccharide |
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| Abstract | The structure of the core region of the lipopolysaccharide (LPS) from the nontypable Haemophilus influenzae strain SB 33 was elucidated. The LPS was subjected to a variety of degradative procedures. The structures of the derived oligosaccharide products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. These analyses revealed a series of related phosphocholine (PCho) containing structures differing in the number of hexose residues. The results pointed to each species containing a conserved phosphoethanolamine (PEtn) substituted heptose-containing trisaccharide inner-core moiety. The major LPS glycoforms were identified as 2-Hex, 3-Hex and 4-Hex species according to the number of hexose residues present |
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| Publication date | 2001-10 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | COX2001 |
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| NPARC number | 9379733 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5b00f96f-e71a-43e4-a944-d0d5055310ca |
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| Record created | 2009-07-10 |
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| Record modified | 2020-03-27 |
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