| Author | Search for: Michon, F.; Search for: Beurret, M.; Search for: Gamian, A.; Search for: Brisson, J.; Search for: Jennings, Harold |
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| Format | Text, Article |
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| Subject | 1H-NMR; acids; assignments; Canada; carbohydrate conformation; carbohydrate sequence; chemical; core oligosaccharide; decasaccharide; hydrolysis; isolation & purification; lipopolysaccharides; magnetic resonance spectroscopy; methylation; molecular sequence data; Neisseria meningitidis; NMR spectroscopy; nuclear; oligosaccharides; one-dimensional; Overhauser; serotype; structure; support, non-U. S. Gov't; triantennary |
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| Abstract | Three different oligosaccharides were isolated by mild acid hydrolysis of the lipopolysaccharides, obtained from Neisseria meningitidis serotype 5, and their structures were elucidated by combined chemical and physical techniques. The use of 500-MHz 1H NMR in both one-dimensional and two-dimensional modes as well as nuclear Overhauser effect experiments were employed. To assist in the structural assignments the purified oligosaccharides were also degraded by chemical and enzymatic procedures to smaller fragments. The largest of the three original oligosaccharides is a triantennary partially O-acetylated decasaccharide in which the largest antenna terminates in a lacto-N-neotetraose unit. The smaller oligosaccharides (heptasaccharide and octasaccharide) except for terminal glycose deletions from the longest antenna are structural replicas of the larger |
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| Publication date | 1990-05-05 |
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| Publisher | American Society for Biochemistry and Molecular Biology |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | MICHON1990 |
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| NPARC number | 9358584 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 573784bc-fb11-49da-8af2-6bd45f039dd9 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-03-17 |
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